Engineering of Effector Domains for Targeted DNA Methylation with Reduced Off-Target Effects
نویسندگان
چکیده
منابع مشابه
Transient overexpression of DNA adenine methylase enables efficient and mobile genome engineering with reduced off-target effects
Homologous recombination of single-stranded oligonucleotides is a highly efficient process for introducing precise mutations into the genome of E. coli and other organisms when mismatch repair (MMR) is disabled. This can result in the rapid accumulation of off-target mutations that can mask desired phenotypes, especially when selections need to be employed following the generation of combinator...
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ژورنال
عنوان ژورنال: International Journal of Molecular Sciences
سال: 2020
ISSN: 1422-0067
DOI: 10.3390/ijms21020502